FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90119
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1250.13870548940844882No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1220.13537655766264606No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1200.13315726983211087No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1190.1320476259168433No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1190.1320476259168433No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1140.12649940634050533No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1140.12649940634050533No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1130.12538976242523772No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1130.12538976242523772No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1120.12428011850997016No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1110.12317047459470257No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1100.12206083067943496No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1100.12206083067943496No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1090.12095118676416737No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1080.11984154284889979No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1060.1176222550183646No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1060.1176222550183646No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1060.1176222550183646No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1050.116512611103097No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1050.116512611103097No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1030.11429332327256184No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1030.11429332327256184No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1020.11318367935729423No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.11096439152675906No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.11096439152675906No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.11096439152675906No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.11096439152675906No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC990.10985474761149147No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.10874510369622388No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.10874510369622388No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.1076354597809563No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.1076354597809563No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.1076354597809563No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.1076354597809563No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.1065258158656887No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.1065258158656887No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC950.1054161719504211No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC950.1054161719504211No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.10430652803515351No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.10430652803515351No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.10319688411988594No Hit
TCGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.10319688411988594No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.10319688411988594No Hit
AACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.10208724020461833No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.10208724020461833No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.10097759628935074No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.10097759628935074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCCT350.0295.000031
TACGCCT350.0295.000031
GCCCCTA650.0295.02
GTGCATC108.4521086E-4295.0295
GCCCCCT157.218281E-6295.01
GTCGCCT157.218281E-6295.01
CGAACCT303.6379788E-12295.01
CGTGCCT255.275069E-10295.01
GCGCCCT157.218281E-6295.01
CTCGCCT303.6379788E-12295.01
GGGCCCT206.153459E-8295.01
CCGACCT206.153459E-8295.01
GGCGCCT157.218281E-6295.01
GATTCCT206.153459E-8295.01
CGATCCT206.153459E-8295.01
CGCCCTA1200.0295.02
CCCGCCT157.218281E-6295.01
GTTGCCT206.153459E-8295.01
TGCCGGG108.4521086E-4295.0295
GGCCTAC3550.0290.845063
Produced by FastQC (version 0.11.5)