FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90119
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16641.8464474750052708No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15261.6933166146983432No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12461.382616318423418No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA12191.352655932711193No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10721.1895382771668572No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA10611.1773321940989137No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9641.0696967343179573No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8850.9820348650118178No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8810.9775962893507474No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8250.9154562300957623No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8110.8999212152820161No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8000.8877151322140725No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7330.8133689898911439No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7160.794505043331595No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7100.7878471798399893No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6850.7601060819582995No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6730.7467903549750885No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6510.7223781888392015No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6420.7123913936017932No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6150.6824310078895682No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5950.6602381295842165No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5880.6524706221773433No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5840.6480320465162729No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5640.6258391682109211No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5190.5759051920238795No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4980.5526026698032601No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4960.550383381972725No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4930.5470544502269222No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4910.544835162396387No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4740.5259712158368379No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4670.5182037084299649No Hit
GGACTACAAGGGTATCTAATCAAGCAGAAGACGGCATACGAGATGACCTG4550.5048879814467537Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4480.49712047403988063No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4450.4937915422940778No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4380.4860240348872047No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4370.4849143909719371No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4360.4838047470566695No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4160.4616118687513177No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4110.45606364917497977No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4060.45051542959864177No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3990.4427479221917686No Hit
GGACTACAAGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATGACCTG3910.43387077086962794Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3900.43276112695436036No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3890.43165148303909273No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3860.42832255129329No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3860.42832255129329No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3650.4050200290726706No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3620.40169109732686775No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3610.40058145341160023No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3590.398362165581065No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3550.39392358991999465No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3550.39392358991999465No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3460.38393679468258635No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3380.3750596433604456No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3320.3684017798688401No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3290.3650728481230373No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3200.355086052885629No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3150.34953783330929106No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3130.34731854547875585No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3120.34620890156348827No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3040.33733175024134754No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2980.330673886749742No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2950.32734495500393923No Hit
GGACTACTAGGGTATCTAATCAAGCAGAAGACGGCATACGAGATGACCTG2880.31957744759706613Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2800.3107002962749254No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2770.3073713645291226No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2750.30515207669858746No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2670.29627492537644673No Hit
GGACTACTAGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATGACCTG2580.28628813013903837Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2570.2851784862237708No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2540.281849554477968No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2500.27741097881689764No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2420.2685338274947569No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2400.26631453966422175No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2390.2652048957489542No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2360.2618759640031514No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2330.2585470322573486No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2300.25521810051154586No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2260.25077952485047544No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2230.2474505931046727No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2220.24634094918940513No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2210.24523130527413753No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2160.23968308569779959No Hit
GGACTACACGGGTATCTAATCAAGCAGAAGACGGCATACGAGATGACCTG2120.2352445100367292Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2110.23413486612146162No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2100.233025222206194No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2100.233025222206194No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2050.22747700262985607No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2020.22414807088405334No Hit
GGACTACACGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATGACCTG1960.21749020739244776Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1920.2130516317313774No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1860.20639376823977187No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1860.20639376823977187No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1840.20417448040923666No Hit
GGACTACTCGGGTATCTAATCAAGCAGAAGACGGCATACGAGATGACCTG1810.20084554866343388Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1760.19529732908709596No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1740.19307804125656078No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1690.1875298216802228No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1670.18531053384968765No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1650.18309124601915244No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1640.18198160210388487No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1630.1808719581886173No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1630.1808719581886173No Hit
GGACTACTCGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATGACCTG1610.17865267035808208Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1580.17532373861227932No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1520.16866587512067377No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1490.165336943374871No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1460.16200801162906822No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1430.15867907988326546No Hit
GGACTACCCGGGTATCTAATCAAGCAGAAGACGGCATACGAGATGACCTG1430.15867907988326546Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1400.1553501481374627No Hit
GGACTACCCGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATGACCTG1340.14869228464585715Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1300.1442537089847868No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1280.1420344211542516No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1280.1420344211542516No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1260.13981513332371642No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1250.13870548940844882No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1250.13870548940844882No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1230.13648620157791366No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1220.13537655766264606No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1220.13537655766264606No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1220.13537655766264606No Hit
GGACTACAGGGGTATCTAATCAAGCAGAAGACGGCATACGAGATGACCTG1210.13426691374737845Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACTGGGGTATCTAATCAAGCAGAAGACGGCATACGAGATGACCTG1210.13426691374737845Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1200.13315726983211087No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1190.1320476259168433No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1180.1309379820015757No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1170.1298283380863081No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1170.1298283380863081No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1160.1287186941710405No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1160.1287186941710405No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1150.12760905025577293No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1130.12538976242523772No Hit
GGACTACCAGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATGACCTG1130.12538976242523772Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1120.12428011850997016No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1110.12317047459470257No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1100.12206083067943496No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1090.12095118676416737No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1090.12095118676416737No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1080.11984154284889979No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1070.1187318989336322No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1070.1187318989336322No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1060.1176222550183646No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1060.1176222550183646No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1040.11540296718782943No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1030.11429332327256184No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1000.11096439152675906No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG990.10985474761149147No Hit
GGACTACAGGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATGACCTG960.1065258158656887Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACCGGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATGACCTG960.1065258158656887Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG950.1054161719504211No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.10430652803515351No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA930.10319688411988594No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA920.10208724020461833No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA920.10208724020461833No Hit
GGACTACTGGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATGACCTG910.10097759628935074Illumina PCR Primer Index 7 (96% over 30bp)
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA910.10097759628935074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTA1800.0294.999458
TACTGGG5800.0294.999455
GGACTAC84000.0294.460481
ACAAGGG20200.0292.808876
TACAAGG20200.0292.808875
GGGGTAT8450.0291.50839
ACCCGGG7900.0291.26536
TACTAGG14150.0290.829835
CAAGGGT20400.0290.661227
CTACTAG14150.0289.787454
TACACGG12200.0288.954385
AGGGTAT19550.0288.209179
TACAGGG6200.0287.862375
ACACGGG12300.0287.804326
CACGGGT12300.0287.804327
GACTACA39050.0287.067322
ACGGGTA5550.0287.02658
ACGGGTT6800.0286.322978
GACTACT31150.0286.00272
AAGGGTA10950.0285.570228
Produced by FastQC (version 0.11.5)