FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77393
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTA1450.0295.000032
TGCGCCT350.0295.000031
GTCCCCT350.0295.000031
TCGCCTA1150.0295.02
GTCGCCT303.6379788E-12295.01
ACGCCTA900.0295.02
CGTGCCT108.449799E-4295.01
GGCACCT255.2568794E-10295.01
ACACCTC108.449799E-4295.02
CCGGCCT206.148002E-8295.01
GGTCCTA1600.0295.02
AGGACCT206.148002E-8295.01
CGCGCCT206.148002E-8295.01
GCGCCTA850.0295.02
GCGCCCT108.449799E-4295.01
CGTTCCT157.2143375E-6295.01
CACCTAG108.449799E-4295.03
CGCCTAG108.449799E-4295.03
TACGCCT206.148002E-8295.01
CGCTCCT108.449799E-4295.01
Produced by FastQC (version 0.11.5)