FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77393
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA15882.051865155763441No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA15782.038944090550825No Hit
GGACTACTCGGGTATCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA12151.5699094233328597No Hit
GGACTACACGGGTATCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA11791.5233935885674414No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA10601.3696329125373097No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA10481.3541276342821702No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10471.3528355277609085No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10291.3295776103781995No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA10241.3231170777718915No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA8971.1590195495716666No Hit
GGACTACTGGGGTATCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA8551.104751075678679No Hit
GGACTACACGGGTTTCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA8031.037561536573075No Hit
GGACTACTCGGGTTTCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA7931.0246404713604589No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA7741.0000904474564885No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7190.9290245887870996No Hit
GGACTACCCGGGTATCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA7110.9186877366170068No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7100.9173956300957451No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7000.9044745648831289No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA6680.8631271562027574No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA6540.8450376649050948No Hit
GGACTACAGGGGTATCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA6200.8011060431821999No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA6170.7972297236184152No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA6110.7894770844908454No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6080.7856007649270605No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA6020.7778481257994909No Hit
GGACTACTGGGGTTTCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA5570.7197033323427183No Hit
GGACTACCGGGGTATCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA5510.7119506932151487No Hit
GGACTACAAGGGTATCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA5240.6770638171410851No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5200.6718953910560387No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5180.6693111780135155No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4960.64088483454576No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4910.6344243019394519No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4900.6331321954181903No Hit
GGACTACCCGGGTTTCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA4810.6215032367268358No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4620.5969532128228652No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4480.5788637215252026No Hit
GGACTACTAGGGTATCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA4350.5620663367488016No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4320.5581900171850168No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4280.5530215910999703No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4160.5375163128448309No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4070.5258873541534764No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3980.5142583954621219No Hit
GGACTACAGGGGTTTCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA3890.5026294367707674No Hit
GGACTACAAGGGTTTCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA3580.46257413461165736No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3550.45869781504787255No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3520.45482149548408773No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3470.44836096287777966No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3390.4380241107076867No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3330.4302714715801171No Hit
GGACTACCGGGGTTTCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA3290.4251030454950706No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3060.39538459550605354No Hit
GGACTACTAGGGTTTCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA3020.39021616942100706No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3010.38892406289974546No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2760.3566213998682051No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2700.34886876074063544No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2690.34757665421937384No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2660.343700334655589No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2600.33594769552801934No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2390.3088134585815255No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2330.3010608194539558No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2180.2816792216350316No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA2180.2816792216350316No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2110.27263447598620033No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2100.2713423694649387No Hit
GGACTACCAGGGTATCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA2020.26100551729484583No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2000.25842130425232257No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1970.25454498468853776No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1900.24550023903970644No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1890.24420813251844484No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1880.2429160259971832No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1840.23774759991213676No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1790.23128706730582868No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1780.22999496078456708No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1750.22611864122078223No Hit
GGACTACCAGGGTTTCTAATCCCGCTCGCTCCCCTAGCTTTCGTCTCTTA1730.223534428178259No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1650.2131975760081661No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1620.20932125644438127No Hit
GGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1600.20673704340185806No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1600.20673704340185806No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1530.19769229775302677No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1450.18735544558293385No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1440.18606333906167224No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1420.183479126019149No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.1808949129766258No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1350.17443438037031772No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1320.1705580608065329No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1310.16926595428527128No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1300.16797384776400967No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1290.16668174124274807No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1280.16538963472148643No Hit
GGACTACACGGGTATCTAATGAGATGGAAGTGGTTGGGGAGGGGACAGGA1270.16409752820022483No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1270.16409752820022483No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1270.16409752820022483No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1240.16022120863644No Hit
GGACTACTCGGGTATCTAATGAGATGGAAGTGGTTGGGGAGGGGACAGGA1240.16022120863644No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1210.15634488907265515No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1200.15505278255139354No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1180.15246856950887033No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1170.1511764629876087No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1150.1485922499450855No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1150.1485922499450855No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1100.1421317173387774No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1060.13696329125373097No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.13696329125373097No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1040.13437907821120773No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1040.13437907821120773No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1040.13437907821120773No Hit
GGACTACACGGGTTTCTAATGAGATGGAAGTGGTTGGGGAGGGGACAGGA1030.13308697168994613No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1010.13050275864742292No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA990.12791854560489968No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA990.12791854560489968No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA980.12662643908363808No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA980.12662643908363808No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG970.12533433256237644No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT960.12404222604111483No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.12404222604111483No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT960.12404222604111483No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC940.1214580129985916No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.1214580129985916No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA930.12016590647732998No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCA930.12016590647732998No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA910.11758169343480676No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA890.11499748039228354No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC880.11370537387102193No Hit
GGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA850.10982905430723709No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA850.10982905430723709No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC840.10853694778597547No Hit
GGACTACTGGGGTATCTAATGAGATGGAAGTGGTTGGGGAGGGGACAGGA840.10853694778597547No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA830.10724484126471386No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT830.10724484126471386No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.10724484126471386No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT820.10595273474345225No Hit
GGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA820.10595273474345225No Hit
GGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA820.10595273474345225No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT820.10595273474345225No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA810.10466062822219063No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA810.10466062822219063No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC810.10466062822219063No Hit
GGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA800.10336852170092903No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA800.10336852170092903No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC790.10207641517966741No Hit
GGACTACTCGGGTTTCTAATGAGATGGAAGTGGTTGGGGAGGGGACAGGA790.10207641517966741No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT780.1007843086584058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)