FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76402
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCT350.0295.000031
CCCTCCT350.0295.000031
AGCCCTA1400.0295.000032
GGATCCT350.0295.000031
CATTCCT350.0295.000031
TATGCCT350.0295.000031
AAGCCTA350.0295.000032
AGACCCT350.0295.000031
AGTTCCT350.0295.000031
TTCGCCT350.0295.000031
CGATCCT350.0295.000031
ATACCTA1450.0295.000032
CACCCTA1550.0295.000032
GCTCCCT350.0295.000031
CTAGCCT350.0295.000031
TAATCCT350.0295.000031
CGGACCT350.0295.000031
TCGCCTA1200.0295.02
GTTGATT206.1474566E-8295.0295
CCTTCCT400.0295.01
Produced by FastQC (version 0.11.5)