FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69356
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1060.1528346502105081No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC970.13985812330584232No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC950.13697445066036104No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.1326489416921391No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.12976526904665783No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.12688159640117652No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.12543976007843588No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.12543976007843588No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.12543976007843588No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.12543976007843588No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.12399792375569527No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.12399792375569527No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.12399792375569527No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.12111425111021397No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.12111425111021397No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.12111425111021397No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.11967241478747333No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.11967241478747333No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.11967241478747333No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.11967241478747333No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.1153469058192514No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.1153469058192514No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.1153469058192514No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.11390506949651076No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.11390506949651076No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.11390506949651076No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.11390506949651076No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.11390506949651076No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.1124632331737701No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.1124632331737701No Hit
GACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11102139685102946No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11102139685102946No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11102139685102946No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11102139685102946No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11102139685102946No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11102139685102946No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.10957956052828884No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.10957956052828884No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.1081377242055482No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.1081377242055482No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.1081377242055482No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.1081377242055482No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.1081377242055482No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.1081377242055482No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10669588788280754No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10669588788280754No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10669588788280754No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.10669588788280754No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1052540515600669No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1052540515600669No Hit
ACCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1052540515600669No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1052540515600669No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.1052540515600669No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10381221523732626No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10381221523732626No Hit
CACGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10381221523732626No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10381221523732626No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10381221523732626No Hit
CCTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10237037891458563No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10237037891458563No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10237037891458563No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10237037891458563No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10237037891458563No Hit
CGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC700.10092854259184497No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC700.10092854259184497No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCT350.0295.000031
TGTTCCT350.0295.000031
AACGCCT350.0295.000031
ACTACCT350.0295.000031
TCTCCCT350.0295.000031
ACTCCTA1450.0295.000032
CGCACCT350.0295.000031
TATCCTA1250.0295.000032
CTAGCCT350.0295.000031
GCCCCTA600.0295.02
GCCCCCT255.2568794E-10295.01
AGTCCTA1100.0295.02
ACGCCTA1300.0295.02
CGGCCTA950.0295.02
CTATCCT303.6379788E-12295.01
AGCCCTA850.0295.02
TGGACCT255.2568794E-10295.01
CTCCCTA850.0295.02
CGACCTA1050.0295.02
GCGCCTA750.0295.02
Produced by FastQC (version 0.11.5)