FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54645
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.17018940433708482No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.1683594107420624No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.16469942355201758No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.1610394363619727No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.15920944276695032No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.15371946198188305No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.15371946198188305No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.15371946198188305No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.15371946198188305No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.15188946838686065No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.15188946838686065No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.15005947479183823No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.1482294811968158No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.1482294811968158No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.1482294811968158No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.1482294811968158No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.1463994876017934No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.1463994876017934No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.1463994876017934No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.1463994876017934No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.144569494006771No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.144569494006771No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.14090950681672615No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.14090950681672615No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.13907951322170373No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.1372495196266813No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.1372495196266813No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.1372495196266813No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.1372495196266813No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.13541952603165888No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.13541952603165888No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.13358953243663646No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.13358953243663646No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.13358953243663646No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.13358953243663646No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.13358953243663646No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.13175953884161407No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.12992954524659164No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.12992954524659164No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.12809955165156922No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.12809955165156922No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.12809955165156922No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.12809955165156922No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.1262695580565468No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.1262695580565468No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.1262695580565468No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.1262695580565468No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.1262695580565468No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC680.12443956446152439No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC680.12443956446152439No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.12260957086650197No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.12260957086650197No Hit
GCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.12260957086650197No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.12260957086650197No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.12260957086650197No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.12260957086650197No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC660.12077957727147956No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC660.12077957727147956No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC660.12077957727147956No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC660.12077957727147956No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC660.12077957727147956No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC660.12077957727147956No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC660.12077957727147956No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC650.11894958367645714No Hit
TGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC650.11894958367645714No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC650.11894958367645714No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC650.11894958367645714No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.11711959008143473No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.11711959008143473No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.11711959008143473No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.11711959008143473No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.11711959008143473No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.11711959008143473No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.11711959008143473No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.11711959008143473No Hit
CCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.1152895964864123No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.1152895964864123No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.1152895964864123No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.1152895964864123No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.1152895964864123No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.1152895964864123No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.1152895964864123No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.1152895964864123No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.11345960289138987No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.11345960289138987No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.11345960289138987No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.11345960289138987No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.11345960289138987No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.11345960289138987No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.11162960929636746No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.11162960929636746No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.11162960929636746No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.11162960929636746No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.11162960929636746No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.11162960929636746No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.11162960929636746No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC610.11162960929636746No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.10979961570134504No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.10979961570134504No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.10979961570134504No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.10979961570134504No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.10979961570134504No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.10979961570134504No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.10979961570134504No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC600.10979961570134504No Hit
GCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC590.10796962210632263No Hit
GGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC590.10796962210632263No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC590.10796962210632263No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC590.10796962210632263No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC590.10796962210632263No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC590.10796962210632263No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC580.10613962851130021No Hit
GAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC580.10613962851130021No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC580.10613962851130021No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC580.10613962851130021No Hit
AATGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.1043096349162778No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.1043096349162778No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.1043096349162778No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.1043096349162778No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC570.1043096349162778No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC560.10247964132125537No Hit
AGCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC560.10247964132125537No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC560.10247964132125537No Hit
TGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC560.10247964132125537No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC560.10247964132125537No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC560.10247964132125537No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.10064964772623297No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.10064964772623297No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.10064964772623297No Hit
CTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.10064964772623297No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.10064964772623297No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.10064964772623297No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.10064964772623297No Hit
GTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.10064964772623297No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC550.10064964772623297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCCT350.0295.000031
CTCCCTA700.0295.000032
CCACGGG350.0295.000035
GCCCCCT303.6379788E-12295.01
GTGCAGT157.2027287E-6295.0295
AATCCTA900.0295.02
GTCGCCT157.2027287E-6295.01
TGCCCCT157.2027287E-6295.01
ACGCCTA850.0295.02
TCACCCT303.6379788E-12295.01
CAGCCTA500.0295.02
GAGTCCT255.2386895E-10295.01
TAGACCT157.2027287E-6295.01
GGGTCCT157.2027287E-6295.01
GATCCTA600.0295.02
TTGTCCT255.2386895E-10295.01
GATCCCT303.6379788E-12295.01
ATAGCCT255.2386895E-10295.01
AGGACCT157.2027287E-6295.01
TGAACCT157.2027287E-6295.01
Produced by FastQC (version 0.11.5)