FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56216
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15892.8265974099900384No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15042.6753949053650206No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14432.5668848726341253No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13332.3712110431193967No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12162.1630852426355487No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10991.9549594421517005No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10941.9460651771737583No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10711.905151558275224No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10691.9015938522840472No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10581.8820264693325743No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10551.876689910345809No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10321.8357762914472748No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10231.8197666144869789No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9191.6347659029457804No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8911.584958019069304No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8171.4533228973957593No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8071.4355343674398748No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8001.4230823964707557No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7821.3910630425501636No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7721.3732745125942791No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7601.3519282766472178No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7031.2505336558986766No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6151.0939945922868934No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6111.0868791803045397No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6091.0833214743133628No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5991.0655329443574784No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5981.06375409136189No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5961.060196385370713No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5871.044186708410417No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5861.0424078554148284No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5550.9872634125515867No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5460.9712537355912907No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5140.9143304397324605No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5100.9072150277501067No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5000.8894264977942222No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4890.8698591148427495No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4630.8236089369574497No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4550.8093781129927422No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4490.7987049950192115No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4300.7649067881030311No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3830.6813006973103742No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3720.6617333143589014No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3570.6350505194250746No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3550.6314928134338978No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3460.6154831364736018No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3420.608367724491248No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3420.608367724491248No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3260.5799060765618329No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3260.5799060765618329No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3190.5674541055927138No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3080.5478867226412409No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2810.4998576917603529No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2710.4820691618044685No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2680.47673260281770313No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2080.3700014230823965No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2070.36822257008680803No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1850.32908780418386224No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1710.304183862245624No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1620.288174185285328No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1600.2846164792941511No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1550.2757222143162089No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1540.27394336132062047No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1420.2525971253735591No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1410.2508182723779707No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1400.24903941938238225No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1380.24548171339120536No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1360.24192400740002845No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1350.24014515440444No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1340.23836630140885157No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1340.23836630140885157No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1330.23658744841326312No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1330.23658744841326312No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1320.23480859541767468No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1300.2312508894264978No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1240.22057777145296714No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1230.21879891845737867No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1210.2152412124662018No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1180.20990465347943643No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1170.20812580048384802No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1100.1956738295147289No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1100.1956738295147289No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1100.1956738295147289No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1090.19389497651914045No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1080.192116123523552No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1070.19033727052796356No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1060.18855841753237512No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1030.1832218585456098No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1030.1832218585456098No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1000.17788529955884447No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA980.17432759356766758No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA940.16721218158531378No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA890.15831791660737157No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA870.1547602106161947No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.1547602106161947No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA860.15298135762060625No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA860.15298135762060625No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA810.144087092642664No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA800.14230823964707556No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.14052938665148712No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA790.14052938665148712No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA770.13697168066031024No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA770.13697168066031024No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA720.128077415682368No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA700.12451970969119112No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA700.12451970969119112No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA700.12451970969119112No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA680.12096200370001423No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA680.12096200370001423No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA670.11918315070442578No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA670.11918315070442578No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA670.11918315070442578No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA660.11740429770883734No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.1156254447132489No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA630.11206773872207201No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA620.11028888572648357No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA620.11028888572648357No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA610.10851003273089513No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA600.10673117973530666No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.10495232673971822No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.10495232673971822No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA580.10317347374412977No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA580.10317347374412977No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA570.10139462074854133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGC550.0295.0295
CTAGGGT5700.0295.07
ACGGGTA3450.0295.08
TAGGGTT3250.0295.08
CTACCGG4350.0295.04
CTACTAG5750.0295.04
TACTAGG5750.0295.05
GACTACT15450.0295.02
CTCCCGG108.443658E-4294.999974
GGACTCC108.443658E-4294.999971
AATTAGC2400.0294.99997295
CAGGCGT108.443658E-4294.999977
ACAGGCG108.443658E-4294.999976
GACTCCC108.443658E-4294.999972
AGTTAGT303.6379788E-12294.99997295
AGTTAGG157.2038547E-6294.99997295
GTTCGCC108.443658E-4294.99997295
GGACTAC52150.0294.717161
GACTACA21350.0294.30912
CGGGTAT8950.0293.351969
Produced by FastQC (version 0.11.5)