FastQCFastQC Report
Wed 8 May 2024
EGAF00005491640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81651
Total Bases24.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6550.8021947067396602No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6160.7544304417582148No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5510.6748233334558058No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5450.6674749849971219No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5420.66380081076778No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5360.656452462309096No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4900.6001151241258527No Hit
GGACTACAAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG4840.5927667756671688No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4700.5756206292635729No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4570.5596992076030912No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4460.5462272354288373No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4340.5315305385114696No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4220.5168338415941017No Hit
GGACTACAAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG4100.502137144676734No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3840.4702943013557703No Hit
GGACTACTAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG3720.45559760443840247No Hit
GGACTACTAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG3580.4384514580348066No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3540.43355255906235074No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3530.43232783431923677No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3290.40293444048450117No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3250.3980355415120452No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3240.39681081676893115No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3160.3870130188240193No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3150.3857882940809053No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3130.3833388445946774No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3080.3772152208791074No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3010.3686421476773095No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3000.36741742293419555No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2970.3637432487048536No Hit
GGACTACCAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2910.3563949002461697No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2840.34782182704437176No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2820.3453723775581438No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2810.34414765281502985No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2660.3257767816683201No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2590.3172037084665221No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2430.2976081125766984No Hit
GGACTACACGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2390.29270921360424246No Hit
GGACTACACGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2360.2890350393749005No Hit
GGACTACCAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG2350.28781031463178647No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2340.2865855898886725No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2290.2804619661731026No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC2210.2706641682281907No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2210.2706641682281907No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2200.26943944348507676No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2180.26698999399884876No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2160.2645405445126208No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2140.26209109502639283No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2140.26209109502639283No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2130.2608663702832788No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC2100.2571921960539369No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2090.25596747131082287No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2060.25229329708148096No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1950.23882132490722713No Hit
GGACTACTCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1950.23882132490722713No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1940.2375966001641131No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1880.2302482517054292No Hit
GGACTACAAGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1860.22779880221920124No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1840.22534935273297324No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1830.22412462798985927No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1810.22167517850363133No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1790.2192257290174033No Hit
GGACTACAAGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1770.21677627953117537No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1770.21677627953117537No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1770.21677627953117537No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1710.20942793107249147No Hit
GGACTACTAGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1710.20942793107249147No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1690.20697848158626347No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1690.20697848158626347No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1680.2057537568431495No Hit
GGACTACTCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1630.19963013312757957No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1630.19963013312757957No Hit
GGACTACTAGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1610.1971806836413516No Hit
GGACTACCCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1590.19473123415512364No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1530.18738288569643974No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1520.18615816095332574No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1510.18493343621021177No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1500.18370871146709777No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1490.18248398672398378No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1470.1800345372377558No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.17880981249464184No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1460.17880981249464184No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1450.17758508775152784No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1450.17758508775152784No Hit
GGACTACAAGGGTATCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT1410.1726861887790719No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1410.1726861887790719No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1380.16901201454972994No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1340.164113115577274No Hit
GGACTACCCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1340.164113115577274No Hit
GGACTACAAGGGTTTCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT1340.164113115577274No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1330.16288839083416004No Hit
GGACTACACGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1290.15798949186170408No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1270.1555400423754761No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1270.1555400423754761No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.1555400423754761No Hit
GGACTACCAGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1240.15186586814613415No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1240.15186586814613415No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1240.15186586814613415No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1240.15186586814613415No Hit
GGACTACTGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1240.15186586814613415No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1230.15064114340302018No Hit
GGACTACCGGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1220.14941641865990618No Hit
GGACTACTAGGGTTTCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT1220.14941641865990618No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1220.14941641865990618No Hit
GGACTACAGGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1210.1481916939167922No Hit
GGACTACAAGGGTATCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT1210.1481916939167922No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1200.1469669691736782No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1190.14574224443056422No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1180.14451751968745025No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1180.14451751968745025No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1170.14329279494433625No Hit
GGACTACTAGGGTATCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT1170.14329279494433625No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1160.14206807020122228No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1160.14206807020122228No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1160.14206807020122228No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1160.14206807020122228No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1150.14084334545810828No Hit
GGACTACACGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1150.14084334545810828No Hit
GGACTACAAGGGTATCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1150.14084334545810828No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1140.1396186207149943No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1110.13594444648565235No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1090.13349499699942438No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1090.13349499699942438No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1090.13349499699942438No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1090.13349499699942438No Hit
GGACTACCAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1090.13349499699942438No Hit
GGACTACTCGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1080.1322702722563104No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1080.1322702722563104No Hit
GGACTACAGGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1070.13104554751319641No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1070.13104554751319641No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1070.13104554751319641No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.12859609802696845No Hit
GGACTACTCGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1050.12859609802696845No Hit
GGACTACCAGGGTTTCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT1040.12737137328385445No Hit
GGACTACTAGGGTATCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT1030.12614664854074048No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1020.12492192379762648No Hit
GGACTACAAGGGTATCTAACATACGTGTACCAGGTTGGCATGCCTCCAGC1010.1236971990545125No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1010.1236971990545125No Hit
GGACTACAAGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1000.12247247431139852No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1000.12247247431139852No Hit
GGACTACTAGGGTTTCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT1000.12247247431139852No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1000.12247247431139852No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA950.1163488505958286No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC950.1163488505958286No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC950.1163488505958286No Hit
GGACTACAAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG940.1151241258527146No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA930.11389940110960062No Hit
GGACTACAAGGGTTTCTAAGTCTGGTCCTTCAACCAAGGGACTGACAACT930.11389940110960062No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC930.11389940110960062No Hit
GGACTACCAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC920.11267467636648662No Hit
GGACTACTGGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG920.11267467636648662No Hit
GGACTACAAGGGTATCTAATACCGGATGTGCTCTGACTCGCCCACTTCCG920.11267467636648662No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC910.11144995162337265No Hit
GGACTACCCGGGTATCTAAGGTGATCAGGCCGCATCAGTCTTCAACCACT900.11022522688025867No Hit
GGACTACAAGGGTTTCTAACATACGTGTACCAGGTTGGCATGCCTCCAGC890.10900050213714467No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC890.10900050213714467No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT880.1077757773940307No Hit
GGACTACACGGGTATCTAATGTGATCAGGCCGCATCAGTCTTCAACCACT870.1065510526509167No Hit
GGACTACAAGGGTTTCTAATACCGGATGTGCTCTGACTCGCCCACTTCCG870.1065510526509167No Hit
GGACTACAAGGGTTTCTAATGGTCCCTGGAGTTTCTCGGCACAGAGCTCA860.10532632790780272No Hit
GGACTACTAGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA860.10532632790780272No Hit
GGACTACAAGGGTATCTAATGGTCCCTGGAGTTTCTCGGCACAGAGCTCA840.10287687842157475No Hit
GGACTACCCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC840.10287687842157475No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA840.10287687842157475No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA840.10287687842157475No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.10287687842157475No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC840.10287687842157475No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.10165215367846077No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC820.10042742893534677No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA820.10042742893534677No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC820.10042742893534677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)