FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81618
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.1127202332818741No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.1114950133548972No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.1114950133548972No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10291847386605896No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10046803401210518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT206.150003E-8295.01
GCCCCTA1100.0295.02
CAAACCT400.0295.01
CGAACCT255.275069E-10295.01
TAGACCT303.6379788E-12295.01
CCGGCCT157.215789E-6295.01
GGTCCCT255.275069E-10295.01
CGCGCCT108.4506476E-4295.01
CGACCCT450.0295.01
GCGCCTA650.0295.02
GCGCCCT255.275069E-10295.01
TAGCCTA1450.0295.02
CGTTCCT400.0295.01
GCACCCT350.0295.01
GTTACCT303.6379788E-12295.01
GTAGCCT450.0295.01
TACGCCT206.150003E-8295.01
CGCTCCT303.6379788E-12295.01
TGCGCCT206.150003E-8295.01
CGGGCGG108.4506476E-4295.08
Produced by FastQC (version 0.11.5)