FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107521
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCT550.0295.000031
AATTCCT550.0295.000031
TACTCCT550.0295.000031
GCATCCT550.0295.000031
TGATCCT450.0295.01
GCCCCTA1600.0295.02
GCCCCCT305.456968E-12295.01
AGTCCTA1750.0295.02
GTCGCCT305.456968E-12295.01
AGTCCCT206.159098E-8295.01
AGATCCT255.275069E-10295.01
ACGCCCT157.2221555E-6295.01
CCCTCCT450.0295.01
GGGCTAC108.4543764E-4295.02
CCTAGGA108.4543764E-4295.05
GAGTCCT400.0295.01
CCCTAAG108.4543764E-4295.04
GTCCTAG108.4543764E-4295.03
GAGTACT157.2221555E-6295.0295
GATCCTA1400.0295.02
Produced by FastQC (version 0.11.5)