FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66607
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC840.1261128710195625No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC830.12461152731694868No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC820.12311018361433482No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC810.12160883991172099No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC800.12010749620910714No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC790.11860615250649331No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC790.11860615250649331No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC780.11710480880387947No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC760.11410212139865178No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC750.11260077769603795No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC750.11260077769603795No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC750.11260077769603795No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC750.11260077769603795No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC740.1110994339934241No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC740.1110994339934241No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC730.10959809029081027No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC730.10959809029081027No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC730.10959809029081027No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC720.10809674658819643No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC720.10809674658819643No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC710.1065954028855826No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC700.10509405918296877No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC700.10509405918296877No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC700.10509405918296877No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC700.10509405918296877No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC700.10509405918296877No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC700.10509405918296877No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC690.10359271548035492No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC690.10359271548035492No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC690.10359271548035492No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC690.10359271548035492No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC690.10359271548035492No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC690.10359271548035492No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC680.1020913717777411No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC680.1020913717777411No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC670.10059002807512724No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC670.10059002807512724No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10059002807512724No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC670.10059002807512724No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC670.10059002807512724No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC670.10059002807512724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCCT350.0295.000031
GGATCCT350.0295.000031
CGAGCCT350.0295.000031
TTCGCCT350.0295.000031
TCGCCTA950.0295.02
TCGCCCT206.141454E-8295.01
AGTCCTA950.0295.02
CCCCTCG108.4471644E-4295.03
TGCCCCT157.209839E-6295.01
GTTTCCT108.4471644E-4295.01
ACCGCCT500.0295.01
CTCCCTA255.2568794E-10295.02
GGTCCTA1150.0295.02
TTGTCCT303.6379788E-12295.01
GTCTCCT450.0295.01
TCCCTAC2750.0295.03
AAGACCT206.141454E-8295.01
CACGCCT157.209839E-6295.01
CCTACGA157.209839E-6295.05
GACCCCT206.141454E-8295.01
Produced by FastQC (version 0.11.5)