FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42055
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC550.13078111996195457No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC550.13078111996195457No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC540.12840328141719176No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.12602544287242898No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.12602544287242898No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.12602544287242898No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.12364760432766615No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.12364760432766615No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.11889192723814053No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.11889192723814053No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.11889192723814053No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.11651408869337772No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.11651408869337772No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.11651408869337772No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.11651408869337772No Hit
ATAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT480.11413625014861492No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC480.11413625014861492No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC470.11175841160385211No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC470.11175841160385211No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC460.10938057305908928No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC460.10938057305908928No Hit
GGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC460.10938057305908928No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC460.10938057305908928No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC460.10938057305908928No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC460.10938057305908928No Hit
TTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT450.10700273451432647No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC450.10700273451432647No Hit
CTTGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC450.10700273451432647No Hit
GAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT440.10462489596956366No Hit
TCTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC440.10462489596956366No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC440.10462489596956366No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC430.10224705742480085No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC430.10224705742480085No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC430.10224705742480085No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC430.10224705742480085No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC430.10224705742480085No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC430.10224705742480085No Hit
ATATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT430.10224705742480085No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC430.10224705742480085No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC430.10224705742480085No Hit
TCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT430.10224705742480085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA950.0295.000032
TGATCCT157.1909635E-6295.01
TCGCCTA650.0295.02
ACAACCT400.0295.01
GTCGCCT206.114715E-8295.01
GTACCCT108.4361027E-4295.01
AGATCCT157.1909635E-6295.01
ACGCCTA550.0295.02
GCGACCT255.2204996E-10295.01
TAGACCT108.4361027E-4295.01
GCAACCT400.0295.01
GATCCTA650.0295.02
CAACCTA1350.0295.02
ATAGCCT206.114715E-8295.01
GGATCCT303.6379788E-12295.01
CCTACGC108.4361027E-4295.05
CGCGAGG108.4361027E-4295.09
CGTTCCT206.114715E-8295.01
GCACCCT157.1909635E-6295.01
TACGCGA108.4361027E-4295.07
Produced by FastQC (version 0.11.5)