FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62520
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC810.1295585412667946No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC780.12476007677543187No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC770.12316058861164428No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC760.1215611004478567No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC750.11996161228406908No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1183621241202815No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11676263595649392No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC720.11516314779270634No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC720.11516314779270634No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC720.11516314779270634No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC700.11196417146513117No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC700.11196417146513117No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC700.11196417146513117No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10876519513755598No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC680.10876519513755598No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC680.10876519513755598No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10716570697376838No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC670.10716570697376838No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC660.10556621880998081No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10556621880998081No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC660.10556621880998081No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC660.10556621880998081No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10396673064619322No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC650.10396673064619322No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC650.10396673064619322No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10396673064619322No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10236724248240564No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC640.10236724248240564No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10236724248240564No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC640.10236724248240564No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10236724248240564No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10076775431861805No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCC630.10076775431861805No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)