FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66712
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11542151337090778No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11542151337090778No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.1094255905983931No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.1094255905983931No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.1094255905983931No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10492864851900709No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.10342966782587841No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC680.10193068713274973No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC670.10043170643962106No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.10043170643962106No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.10043170643962106No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.10043170643962106No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.10043170643962106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATT350.0295.00003295
AATCCTA1400.0295.000032
GAAGCCT350.0295.000031
CGCTCCT350.0295.000031
TAGCCCT350.0295.000031
ATTACCT350.0295.000031
CCACCCT350.0295.000031
TCTGCCT350.0295.000031
GCACCCT350.0295.000031
ATCGCCT350.0295.000031
AGATCCT350.0295.000031
CGATCCT350.0295.000031
ATCCCCT157.2098883E-6295.01
AGCCCCT255.2568794E-10295.01
ATCCTCG206.141636E-8295.03
TTGTCCT400.0295.01
ACGCCTA900.0295.02
ACGCCCT303.6379788E-12295.01
GCCTCCT157.2098883E-6295.01
GTCACCT450.0295.01
Produced by FastQC (version 0.11.5)