FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74795
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11631793569088843No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11364396015776455No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10695902132495487No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10428504579183101No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10161107025870714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCTA1250.0295.000032
TCACCTA1250.0295.000032
ATACCTA1250.0295.000032
GCCCTCG108.44923E-4295.03
CGTTCCT157.213368E-6295.01
ATCCCCT303.6379788E-12295.01
AGCCCCT255.2568794E-10295.01
TTGTCCT550.0295.01
TGGTCCT400.0295.01
TCCTCGG108.44923E-4295.04
AACGCCT303.6379788E-12295.01
ACGCCTA1150.0295.02
ACGCTTT108.44923E-4295.0295
TTCACCT206.146547E-8295.01
TGCACCT350.0295.01
GGGTCCT157.213368E-6295.01
AATCCCT255.2568794E-10295.01
GCCTCCT450.0295.01
GTCACCT350.0295.01
GGCACCT108.44923E-4295.01
Produced by FastQC (version 0.11.5)