FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74795
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA23653.1619760679189786No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA19242.5723644628651647No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA18332.450698576108029No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA15862.1204625977672307No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA14791.9774049067451034No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA14201.8985226285179493No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13681.8289992646567284No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13531.8089444481582992No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13151.758138913028946No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12781.7086703656661544No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11831.58165652784277No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11711.565612674644027No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10911.4586536533190722No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10861.4519687144862625No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10211.3650645096597367No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10201.3637275218931746No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10161.3583795708269268No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9751.3035630723978877No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9341.2487465739688481No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8931.1939300755398088No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8811.1778862223410655No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8721.1658533324420082No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8591.148472491476703No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8381.1203957483789022No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8321.1123738217795307No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8151.0896450297479778No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7601.0161107025870713No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7470.9987298616217662No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7420.9920449227889564No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7220.9653051674577178No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6950.9292064977605454No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6410.857009158366201No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6140.8209104886690287No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5790.7741159168393609No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5680.7594090514071796No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5510.7366802593756266No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5260.7032555652115784No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4740.6337322013503576No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4660.6230362992178622No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4540.606992446019119No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4310.5762417273881943No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4180.5588608864228892No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4150.5548499231232035No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3910.522762216725717No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3440.459923791697306No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3270.4371949996657531No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3060.40911825656795237No Hit
GGACTACAAGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3040.40644428103482855No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2960.39574837890233305No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2800.37435657463734207No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2790.37301958687078013No Hit
GGACTACAAGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2720.36366067250484657No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2700.36098669697172275No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2640.3529647703723511No Hit
GGACTACTAGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2350.31419212514205497No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2310.3088441740758072No Hit
GGACTACAAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2180.29146333311050204No Hit
GGACTACAAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2130.28477839427769236No Hit
GGACTACAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2120.2834414065111304No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2020.27007152884551106No Hit
GGACTACTAGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2000.2673975533123872No Hit
GGACTACACGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1920.2567016511798917No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1840.24600574904739622No Hit
GGACTACTAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1830.24466876128083426No Hit
GGACTACAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1790.23932081021458657No Hit
GGACTACAAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1720.229961895848653No Hit
GGACTACACGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1720.229961895848653No Hit
GGACTACAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1670.2232769570158433No Hit
GGACTACTAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1620.21659201818303364No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1620.21659201818303364No Hit
GGACTACTCGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1620.21659201818303364No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1520.20322214051741427No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1510.20188515275085234No Hit
GGACTACCAGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1490.19921117721772846No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1480.19787418945116653No Hit
GGACTACTGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1460.19520021391804265No Hit
GGACTACTGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1450.19386322615148072No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA1380.18450431178554716No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1380.18450431178554716No Hit
GGACTACACGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1360.18183033625242329No Hit
GGACTACTGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1240.16578648305368004No Hit
GGACTACCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1210.16177551975399426No Hit
GGACTACCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1200.1604385319874323No Hit
GGACTACAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1190.1591015442208704No Hit
GGACTACCAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1180.15776455645430845No Hit
GGACTACTAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1160.15509058092118458No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA1150.15375359315462261No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1150.15375359315462261No Hit
GGACTACTCGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1140.1524166053880607No Hit
GGACTACCAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1140.1524166053880607No Hit
GGACTACTGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1100.14706865432181296No Hit
GGACTACCCGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1100.14706865432181296No Hit
GGACTACCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1080.14439467878868908No Hit
GGACTACCAGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1060.1417207032555652No Hit
GGACTACACGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1060.1417207032555652No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1050.14038371548900327No Hit
GGACTACTCGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG1030.1377097399558794No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1030.1377097399558794No Hit
GGACTACACGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1030.1377097399558794No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA1020.13637275218931746No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1020.13637275218931746No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA1020.13637275218931746No Hit
GGACTACAAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1020.13637275218931746No Hit
GGACTACCCGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA990.13236178888963165No Hit
GGACTACTCGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA970.12968781335650778No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA960.12835082558994584No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA950.1270138378233839No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA930.12433986229026005No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA920.12300287452369811No Hit
GGACTACCAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA910.12166588675713617No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA900.12032889899057422No Hit
GGACTACTAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA870.11631793569088843No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA870.11631793569088843No Hit
GGACTACCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA860.1149809479243265No Hit
GGACTACAGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA840.11230697239120262No Hit
GGACTACTGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA830.1109699846246407No Hit
GGACTACAAGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA790.10562203355839295No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA790.10562203355839295No Hit
GGACTACCCGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG790.10562203355839295No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA790.10562203355839295No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA780.10428504579183101No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA780.10428504579183101No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA760.10161107025870714No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA760.10161107025870714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)