FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51831
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1215488800138913No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC610.1176901854102757No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC590.11383149080666012No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.11383149080666012No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC580.11190214350485231No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC580.11190214350485231No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.11190214350485231No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC580.11190214350485231No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10997279620304451No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC560.10804344890123672No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC550.10611410159942891No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC540.10418475429762111No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC540.10418475429762111No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC520.1003260596940055No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.1003260596940055No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.1003260596940055No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC520.1003260596940055No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.1003260596940055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC3550.0295.000033
CGTCCTA850.0295.000032
CGTTCCT255.2386895E-10295.01
AATCCTA750.0295.02
ACGCCTA550.0295.02
TGACCTA1500.0295.02
TCGACCT255.2386895E-10295.01
GCGACCT255.2386895E-10295.01
GGTCCTA1100.0295.02
GAGCCTA500.0295.02
GGATCCT350.0295.01
ACACCCT255.2386895E-10295.01
CGGACCT255.2386895E-10295.01
CCCACCT255.2386895E-10295.01
TAGTCCT350.0295.01
TCTACCT255.2386895E-10295.01
CTAGGGA450.0295.06
CCACCTA900.0295.02
TGCCTAC2150.0295.03
CACCCTA900.0295.02
Produced by FastQC (version 0.11.5)