FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135260
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC2040.1508206417270442No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC2010.1486026911134112No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1960.14490610675735618No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1930.1426881561437232No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1840.1360343043028242No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1840.1360343043028242No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1830.1352949874316132No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1800.1330770368179802No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1800.1330770368179802No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1740.12864113559071416No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1720.12716250184829217No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1700.12568386810587018No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1680.12420523436344817No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1670.12346591749223716No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1670.12346591749223716No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1670.12346591749223716No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1660.12272660062102618No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1630.12050865000739316No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1630.12050865000739316No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1630.12050865000739316No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1620.11976933313618218No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1620.11976933313618218No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1610.11903001626497117No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1610.11903001626497117No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1600.11829069939376016No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1600.11829069939376016No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1570.11607274878012716No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1560.11533343190891615No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1560.11533343190891615No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1550.11459411503770517No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1550.11459411503770517No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1550.11459411503770517No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1550.11459411503770517No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1540.11385479816649416No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1540.11385479816649416No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1540.11385479816649416No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1530.11311548129528316No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1520.11237616442407215No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1520.11237616442407215No Hit
GGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1510.11163684755286114No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1510.11163684755286114No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1510.11163684755286114No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1510.11163684755286114No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1500.11089753068165016No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1490.11015821381043915No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1490.11015821381043915No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1490.11015821381043915No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1480.10941889693922816No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1480.10941889693922816No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1470.10867958006801715No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1460.10794026319680616No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1460.10794026319680616No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1460.10794026319680616No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1450.10720094632559515No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1440.10646162945438416No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1440.10646162945438416No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1440.10646162945438416No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1440.10646162945438416No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1430.10572231258317315No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1420.10498299571196215No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1420.10498299571196215No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1410.10424367884075114No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1410.10424367884075114No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1400.10350436196954013No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1400.10350436196954013No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1400.10350436196954013No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1400.10350436196954013No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1390.10276504509832915No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1390.10276504509832915No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1390.10276504509832915No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1390.10276504509832915No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1370.10128641135590714No Hit
AGACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1370.10128641135590714No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1370.10128641135590714No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1370.10128641135590714No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1360.10054709448469613No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1360.10054709448469613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCTA1900.0295.000032
TGATCCT500.0295.01
TCGCCTA1750.0295.02
TCTGCCT700.0295.01
GCCCCTA1250.0295.02
AGTCCTA2550.0295.02
AGCAAAT157.2262737E-6295.0295
ATCTCCT500.0295.01
GTCGCCT255.293259E-10295.01
CCCCTAC7450.0295.03
TGCCCCT157.2262737E-6295.01
AGATCCT600.0295.01
CCCTCCT600.0295.01
GTACAGT206.164919E-8295.0295
CAACGGG108.4567856E-4295.06
CTTCCCT350.0295.01
AGCCCTA1450.0295.02
GAGTCCT500.0295.01
TAGACCT500.0295.01
GGGTCCT500.0295.01
Produced by FastQC (version 0.11.5)