FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77094
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC850.11025501336031339No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC790.10247230653487949No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.10247230653487949No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1000.0295.02
GTTGATT400.0295.0295
GCCCCTA1350.0295.02
ACAACCT350.0295.01
TTGGCCT350.0295.01
GTCGCCT255.2568794E-10295.01
AGATCCT206.1478204E-8295.01
CGAACCT255.2568794E-10295.01
CCCTCGG206.1478204E-8295.04
GCGACCT550.0295.01
TCCGCCT206.1478204E-8295.01
CAACGGG108.4497355E-4295.06
TTAGCCT255.2568794E-10295.01
TGGACCT206.1478204E-8295.01
GGCACCT400.0295.01
ACACCTA1400.0295.02
GGTCCTA550.0295.02
TTGTCCT400.0295.01
GATCCCT500.0295.01
CAACCTA1350.0295.02
Produced by FastQC (version 0.11.5)