FastQCFastQC Report
Wed 8 May 2024
EGAF00005491685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64388
Total Bases19.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC810.12579983847921972No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.11492824749953408No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC730.1133751630738647No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC690.10716282537118718No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.10716282537118718No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.10560974094551781No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10405665651984841No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10405665651984841No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10405665651984841No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC660.10250357209417903No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC660.10250357209417903No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC660.10250357209417903No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC650.10095048766850966No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)