FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64388
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC810.12579983847921972No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.11492824749953408No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC730.1133751630738647No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC690.10716282537118718No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.10716282537118718No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.10560974094551781No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10405665651984841No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10405665651984841No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10405665651984841No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC660.10250357209417903No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC660.10250357209417903No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC660.10250357209417903No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCC650.10095048766850966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCT350.0295.000031
CGAACCT350.0295.000031
GGTCCCT350.0295.000031
GCACCCT350.0295.000031
CGATCCT350.0295.000031
ATCGCCT350.0295.000031
TGATCCT255.2568794E-10295.01
GCCCCTA400.0295.02
GTACCTA800.0295.02
GTACCCT255.2568794E-10295.01
CAAACCT600.0295.01
ACGCCTA750.0295.02
CAACGGG108.446502E-4295.06
GAGTCCT255.2568794E-10295.01
GGCACCT157.2087078E-6295.01
CCGGCCT303.6379788E-12295.01
GATCCTA750.0295.02
CTCCCTA1200.0295.02
TTGTCCT255.2568794E-10295.01
GATCCCT157.2087078E-6295.01
Produced by FastQC (version 0.11.5)