FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41481
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.17598418553072492No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC640.15428750512282732No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.15187676285528315No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.15187676285528315No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC600.14464453605265062No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC580.13982305151756225No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC560.1350015669824739No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC560.1350015669824739No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC560.1350015669824739No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC560.1350015669824739No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC550.13259082471492972No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC550.13259082471492972No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC540.13018008244738555No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC540.13018008244738555No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC530.12776934017984137No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC530.12776934017984137No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC530.12776934017984137No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.1253585979122972No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.1253585979122972No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.1253585979122972No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC510.12294785564475302No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC510.12294785564475302No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC510.12294785564475302No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC510.12294785564475302No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC500.12053711337720885No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC500.12053711337720885No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.11812637110966467No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.11812637110966467No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.11812637110966467No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.11571562884212047No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.11571562884212047No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.11571562884212047No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.11330488657457631No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.11330488657457631No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.11330488657457631No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.11330488657457631No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.11330488657457631No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC460.11089414430703214No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC460.11089414430703214No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC460.11089414430703214No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC460.11089414430703214No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC450.10848340203948796No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC450.10848340203948796No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC450.10848340203948796No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC450.10848340203948796No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC450.10848340203948796No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC450.10848340203948796No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC450.10848340203948796No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
ATGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
GTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC440.10607265977194379No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
CATGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC430.10366191750439961No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC420.10125117523685542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)