FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75911
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1190.1567625245353111No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1170.15412786025740668No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1150.1514931959795023No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1100.14490653528474134No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1080.14227187100683694No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1070.14095453886788478No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1070.14095453886788478No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1070.14095453886788478No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1060.13963720672893257No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1040.13700254245102816No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.13568521031207598No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.13568521031207598No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.13568521031207598No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.13568521031207598No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1020.1343678781731238No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1000.1317332138952194No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC990.1304158817562672No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC990.1304158817562672No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC990.1304158817562672No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC980.129098549617315No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC970.12778121747836282No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC960.12646388533941064No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC960.12646388533941064No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC950.12514655320045842No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC950.12514655320045842No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC950.12514655320045842No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC950.12514655320045842No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC940.12382922106150623No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.12119455678360186No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.12119455678360186No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.12119455678360186No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.11987722464464966No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.11987722464464966No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.11987722464464966No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.11987722464464966No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC900.11855989250569746No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC900.11855989250569746No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.11855989250569746No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC890.11724256036674528No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC890.11724256036674528No Hit
GGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC890.11724256036674528No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC890.11724256036674528No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC880.11592522822779308No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC880.11592522822779308No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC880.11592522822779308No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC870.11460789608884088No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC870.11460789608884088No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.11460789608884088No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC870.11460789608884088No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC870.11460789608884088No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC870.11460789608884088No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC860.11329056394988868No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC860.11329056394988868No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC860.11329056394988868No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC860.11329056394988868No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.11329056394988868No Hit
GACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC860.11329056394988868No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC850.11197323181093648No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.11197323181093648No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC850.11197323181093648No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC850.11197323181093648No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC850.11197323181093648No Hit
CGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC850.11197323181093648No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC850.11197323181093648No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC850.11197323181093648No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC840.1106558996719843No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC840.1106558996719843No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC840.1106558996719843No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC840.1106558996719843No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC840.1106558996719843No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC830.1093385675330321No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC830.1093385675330321No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC830.1093385675330321No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC830.1093385675330321No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC830.1093385675330321No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC830.1093385675330321No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC820.1080212353940799No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC820.1080212353940799No Hit
TCGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC820.1080212353940799No Hit
CGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC810.10670390325512771No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC810.10670390325512771No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.10670390325512771No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC810.10670390325512771No Hit
GGGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC810.10670390325512771No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC810.10670390325512771No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC810.10670390325512771No Hit
AGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC810.10670390325512771No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC810.10670390325512771No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC800.10538657111617553No Hit
TACGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC800.10538657111617553No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.10538657111617553No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC800.10538657111617553No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.10538657111617553No Hit
GTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC800.10538657111617553No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC800.10538657111617553No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10406923897722334No Hit
GCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC790.10406923897722334No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC790.10406923897722334No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC790.10406923897722334No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC790.10406923897722334No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10406923897722334No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC790.10406923897722334No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC790.10406923897722334No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC780.10275190683827114No Hit
GTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC780.10275190683827114No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10275190683827114No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10275190683827114No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC780.10275190683827114No Hit
GGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC780.10275190683827114No Hit
GGCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC770.10143457469931894No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC770.10143457469931894No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC770.10143457469931894No Hit
TTTGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC770.10143457469931894No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.10143457469931894No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.10143457469931894No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC760.10011724256036673No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC760.10011724256036673No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.10011724256036673No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC760.10011724256036673No Hit
ATAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC760.10011724256036673No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC760.10011724256036673No Hit
GCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC760.10011724256036673No Hit
CTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC760.10011724256036673No Hit
CTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC760.10011724256036673No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC760.10011724256036673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACCT350.0295.000031
GGCACCT350.0295.000031
GCGCCTA700.0295.000032
AGGGTCG350.0295.00003295
CCGTCCT350.0295.000031
AAGCCTA700.0295.000032
AGTGCCT350.0295.000031
TACGCCT350.0295.000031
TCGACCT350.0295.000031
TCGCCTA450.0295.02
TGCCCTA750.0295.02
ACGCCTA1250.0295.02
CGTGCCT108.449486E-4295.01
GAGTCCT303.6379788E-12295.01
GGGTCCT303.6379788E-12295.01
GTCCTAG157.2138046E-6295.03
GGTCCTA1500.0295.02
TTGTCGG108.449486E-4295.0295
GATCCCT206.147093E-8295.01
GTCTCCT255.2568794E-10295.01
Produced by FastQC (version 0.11.5)