FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107425
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1100.10239702117756574No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1100.10239702117756574No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1090.1014661391668606No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1090.1014661391668606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCCT700.0294.996771
GCACCTA1950.0294.996772
TCGTCCT700.0294.996771
ACTCCCT350.0294.996771
TAGTCCT350.0294.996771
TGGTCCT700.0294.996771
TGATCCT255.275069E-10294.996731
GCCCCTA1500.0294.996732
GCCCCCT305.456968E-12294.996731
ATCCTCG108.454644E-4294.996733
GTCGCCT255.275069E-10294.996731
TGCCCCT600.0294.996731
GCGACCT600.0294.996731
GTCGATA108.454644E-4294.99673295
GGGTCTA108.454644E-4294.996731
GAGTCCT550.0294.996731
CTCCCTA1200.0294.996732
GTCTCCT206.15928E-8294.996731
GGTCCCT255.275069E-10294.996731
AAGACCT550.0294.996731
Produced by FastQC (version 0.11.5)