FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99355
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCT400.0295.01
CGACCTC108.4534124E-4295.0295
CCTACGA157.220511E-6295.05
CGTTCCT350.0295.01
GTTACCT206.1567334E-8295.01
TACGCCT305.456968E-12295.01
TGCGCCT400.0295.01
GTGTCGG157.220511E-6295.0295
TATTCCT450.0295.01
GAGCCTA850.0295.02
GTCCCTA1250.0295.02
GACGCCT305.456968E-12295.01
CGGTCCT350.0295.01
GGCGCCT108.4534124E-4295.01
ATCGCCT500.0295.01
GACCTAC6400.0292.69533
CGCCTAC4350.0291.60923
GCCTACG17300.0291.58964
ACCTACG26700.0291.132974
CCTACGG94850.0290.334755
Produced by FastQC (version 0.11.5)