FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78442
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.12365824430789628No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.12365824430789628No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC950.12110858978608399No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.1134596262206471No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.11090997169883482No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.11090997169883482No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.10963514443792866No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.1083603171770225No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.1083603171770225No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.10708548991611637No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.10581066265521022No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.10581066265521022No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.10581066265521022No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10453583539430408No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10453583539430408No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10326100813339792No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.10071135361158563No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.10071135361158563No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.10071135361158563No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.10071135361158563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTAC550.0295.00003295
CTCGCCT550.0295.000031
TCGCCTA1150.0295.02
TGGACCT206.148366E-8295.01
GAGTACG157.2147104E-6295.0295
GCGCCTA850.0295.02
GTAGCCT450.0295.01
TACGCCT303.6379788E-12295.01
CTACGGC108.450016E-4295.06
TTGCCTA950.0295.02
GACGCCT206.148366E-8295.01
GGCGCCT157.2147104E-6295.01
GTCGGAG108.450016E-4295.0295
GTTGCCT108.450016E-4295.01
CGGACCT255.275069E-10295.01
CGCCTAC4250.0291.529423
TACGGGA71500.0289.22387
CTACGGG72550.0289.104066
CCTACGG74850.0289.088175
CCCTACG18600.0288.65594
Produced by FastQC (version 0.11.5)