FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56566
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA600.0295.02
GTCGCCT157.204091E-6295.01
ACGCCTA500.0295.02
AGCCCTA450.0295.02
CTCCCTA900.0295.02
GGTCCTA850.0295.02
CGCGCCT255.2386895E-10295.01
ATCCCCT303.6379788E-12295.01
AACGCCT157.204091E-6295.01
CCCCCTA850.0295.02
GAGCCTA350.0295.02
CGCACCT157.204091E-6295.01
CGTCCTA1000.0295.02
TAAGCCT350.0295.01
CGATCCT255.2386895E-10295.01
GTGCCTA600.0295.02
AGATCCT206.1334504E-8294.999971
CGAACCT206.1334504E-8294.999971
GGTCCCT108.443797E-4294.999971
GCGCCTA800.0294.999972
Produced by FastQC (version 0.11.5)