FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69748
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.1376383552216551No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.1304696908871939No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.11756609508516372No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1089636978838103No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.10752996501691804No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.10466249928313356No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10322876641624132No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10322876641624132No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10322876641624132No Hit
AGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1017950335493491No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10036130068245684No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10036130068245684No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10036130068245684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA750.0295.02
TTCTACG108.4480067E-4295.03
TGCCCCT303.6379788E-12295.01
ACGCCTA550.0295.02
GCGACCT255.2568794E-10295.01
TTAGCCT450.0295.01
ACCGCCT650.0295.01
CAACCTA1800.0295.02
CACGCCT255.2568794E-10295.01
CGCGCCT108.4480067E-4295.01
CCTACGA157.211278E-6295.05
AAGCCTA750.0295.02
TGCGCCT157.211278E-6295.01
TCTACGG206.143637E-8295.04
CTACGAG157.211278E-6295.06
AGCGCCT206.143637E-8295.01
GGGCCTA700.0295.02
CGTACCT350.0295.01
TACTCCT450.0295.01
ACGTCCT400.0295.01
Produced by FastQC (version 0.11.5)