FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89148
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCT450.0295.01
GCACCTA1850.0295.02
TACGCCT400.0295.01
GACGCCT108.4519514E-4295.01
GGCGCCT206.1530955E-8295.01
CTACGAG157.2180137E-6294.999976
GCTACGG157.2180137E-6294.999974
GCCTACG14050.0290.800724
GTCCTAC5700.0289.824553
CCCTACG19000.0289.56584
TCCTACG24200.0289.514434
CCTACGG80450.0289.316355
CTACGGG79250.0287.927436
GGTCCTA1800.0286.805572
ACGGGAG78900.0286.587468
TACGGGA78850.0286.582127
GCCCTAC4750.0285.684233
ACCTACG23550.0285.60514
CGCCTAC3900.0283.653873
CGTCCTA1200.0282.70832
Produced by FastQC (version 0.11.5)