FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76812
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA1250.0295.02
CTGTCCT157.2141374E-6295.01
GTCGCCT255.2568794E-10295.01
AGATCCT157.2141374E-6295.01
CGAACCT303.6379788E-12295.01
TGGACCT303.6379788E-12295.01
ATTACCT303.6379788E-12295.01
TTGTCCT350.0295.01
ATAGCCT255.2568794E-10295.01
CGCGCCT255.2568794E-10295.01
GGATCCT303.6379788E-12295.01
GCGCCTA700.0295.02
CCTACGA157.2141374E-6295.05
GCGCCCT350.0295.01
GTTACCT255.2568794E-10295.01
ATTCCCT350.0295.01
TTCCCTA1150.0295.02
TACGCCT255.2568794E-10295.01
CTACGAG157.2141374E-6295.06
CGGGCCT157.2141374E-6295.01
Produced by FastQC (version 0.11.5)