FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71768
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA850.0295.000032
CCTACCT350.0295.000031
GAGCCTA700.0295.000032
CGGTCCT350.0295.000031
GATTCCT350.0295.000031
TTCGCCT350.0295.000031
TGATCCT650.0295.01
TCGCCCT255.2568794E-10295.01
GATACCT157.212162E-6295.01
GTCGCCT157.212162E-6295.01
TGCCCCT450.0295.01
AGATCCT157.212162E-6295.01
CGAACCT303.6379788E-12295.01
GAGTCCT157.212162E-6295.01
GGCACCT303.6379788E-12295.01
GAGTATC157.212162E-6295.0295
GATCCCT303.6379788E-12295.01
GTCTCCT550.0295.01
CACGCCT255.2568794E-10295.01
CGCGCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)