FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69311
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10099407020530651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCTA1400.0295.000032
AAGACCT350.0295.000031
TAGCCCT350.0295.000031
ATTCCTA1400.0295.000032
AGGCCTA650.0295.000032
ACATCCT350.0295.000031
GCCACCT350.0295.000031
TAGTCCT350.0295.000031
TGATCCT157.2110997E-6295.01
TCGCCTA900.0295.02
GTTGATT157.2110997E-6295.0295
TCTGCCT400.0295.01
GCCCCTA850.0295.02
GCCCCCT108.447903E-4295.01
CGGCGGC108.447903E-4295.09
GTCGCCT400.0295.01
TGCCCCT206.143273E-8295.01
AGATCCT255.2568794E-10295.01
CCCTCCT108.447903E-4295.01
CCCTCCG108.447903E-4295.03
Produced by FastQC (version 0.11.5)