FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70245
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA700.0295.000032
CAGCCTA550.0295.000032
CGGCCTA700.0295.000032
GAGTCCT350.0295.000031
GACACCT350.0295.000031
CCGGCCT350.0295.000031
CTCCCTA1100.0295.000032
AGGACCT350.0295.000031
TATTCCT350.0295.000031
AGACCCT350.0295.000031
GGACCTA1100.0295.000032
GTGACCT350.0295.000031
CGATCCT350.0295.000031
GCATCCT350.0295.000031
TGATCCT400.0295.01
TCGCCTA600.0295.02
GCCCCCT157.2115E-6295.01
CGGCGGC206.1438186E-8295.09
GTCGCCT157.2115E-6295.01
ACGCCTA750.0295.02
Produced by FastQC (version 0.11.5)