FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102654
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1270.12371656243302745No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1190.1159233931459076No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1190.1159233931459076No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1180.11494924698501763No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1100.1071560776978978No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1060.10325949305433786No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1060.10325949305433786No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1050.1022853468934479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCCT305.456968E-12294.99661
GCGCGAA157.2215407E-6294.9966295
CGTTCCT550.0294.99661
TTCCCCT550.0294.99661
ATCCCTA1200.0294.99662
TATTCCT600.0294.99661
GGGCCCT305.456968E-12294.99661
CCGACCT305.456968E-12294.99661
GGCGCCT305.456968E-12294.99661
CGCACCT550.0294.99661
CGATCCT550.0294.99661
CGCCCTA1100.0294.99662
GGCTCCT157.2215407E-6294.99661
TATCCCT305.456968E-12294.99661
TCGCCTA1250.0294.996582
TCTGCCT206.158007E-8294.996581
GCCCCCT108.4541144E-4294.996581
GTCGCCT255.275069E-10294.996581
TGCCCCT450.0294.996581
CGAACCT255.275069E-10294.996581
Produced by FastQC (version 0.11.5)