FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61801
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.1456287115095225No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.14239251792042198No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.14077442112587177No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.14077442112587177No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.13753822753677125No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.13106584035857025No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.13106584035857025No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12944774356402No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12944774356402No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12944774356402No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.12782964676946976No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1262115499749195No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1262115499749195No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1262115499749195No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.1262115499749195No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12459345318036924No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12459345318036924No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12459345318036924No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12459345318036924No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.12297535638581901No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.12297535638581901No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.12297535638581901No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12135725959126875No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12135725959126875No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1197391627967185No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1197391627967185No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11812106600216825No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11812106600216825No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11812106600216825No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11812106600216825No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11812106600216825No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11650296920761799No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11650296920761799No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11650296920761799No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11650296920761799No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11650296920761799No Hit
ACAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11488487241306776No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11488487241306776No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC710.11488487241306776No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1132667756185175No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1132667756185175No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1132667756185175No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.1132667756185175No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1132667756185175No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1132667756185175No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.1132667756185175No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.1132667756185175No Hit
AAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11164867882396724No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11164867882396724No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11164867882396724No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.110030582029417No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC680.110030582029417No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.110030582029417No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.110030582029417No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC680.110030582029417No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10841248523486674No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10841248523486674No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10841248523486674No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10841248523486674No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.10841248523486674No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC670.10841248523486674No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10841248523486674No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10679438844031651No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10679438844031651No Hit
TCGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10679438844031651No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC660.10679438844031651No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10679438844031651No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10679438844031651No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10517629164576625No Hit
CTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10517629164576625No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10517629164576625No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC650.10517629164576625No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10517629164576625No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.10355819485121599No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10355819485121599No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10355819485121599No Hit
TATCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10355819485121599No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10355819485121599No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10355819485121599No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC640.10355819485121599No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10194009805666576No Hit
TTGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10194009805666576No Hit
CGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10194009805666576No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC630.10194009805666576No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10194009805666576No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10194009805666576No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10194009805666576No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10032200126211549No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.10032200126211549No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10032200126211549No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC620.10032200126211549No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10032200126211549No Hit
TTTGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10032200126211549No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10032200126211549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCTA950.0295.000032
CGTCCTA950.0295.000032
AGCCTAC3100.0295.03
GTTGATT206.137998E-8295.0295
TTGTCCT450.0295.01
TCCTCGG157.207327E-6295.04
TGCACCT157.207327E-6295.01
CCAACCT600.0295.01
ACGCCCT108.445692E-4295.01
CCTACGA206.137998E-8295.05
CAGTCCT157.207327E-6295.01
GTCACCT400.0295.01
ATCTCCT400.0295.01
AGCTCCT350.0295.01
TATACCT206.137998E-8295.01
TCGACCT450.0295.01
CGCCCTA650.0295.02
TTCGCCT157.207327E-6295.01
TGCGCCT206.137998E-8295.01
TTACCCT400.0295.01
Produced by FastQC (version 0.11.5)