FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114797
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCCT206.161099E-8294.996981
ACGGGCG108.455379E-4294.996988
TTACGGG108.455379E-4294.996986
GACGCCT206.161099E-8294.996981
CTTACGG108.455379E-4294.996985
TGATCCT450.0294.996951
TCGCCTA1650.0294.996952
TCGCCCT750.0294.996951
TACGGGC157.2237235E-6294.996957
TGACGCC157.2237235E-6294.99695295
GCACCCT350.0294.996951
GGCGCCT350.0294.996951
ATCGCCT350.0294.996951
CGCCCTA1450.0294.996952
GCCTACG21250.0292.220524
CCTACGG107150.0289.903665
CTACGGG105150.0288.68466
TCCTACG31700.0288.482824
ACCTACG31300.0288.39964
ACGGGAG103950.0287.33478
Produced by FastQC (version 0.11.5)