FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99822
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCT350.0295.01
GCGACCT350.0295.01
CAACGGG157.2206094E-6295.06
CGCGCCT255.275069E-10295.01
GGATCCT400.0295.01
GCGCCTA900.0295.02
CCTACGA108.4534695E-4295.05
CGTTCCT700.0295.01
ACGGGAC108.4534695E-4295.08
AACGGGA157.2206094E-6295.07
CGGGGCA157.2206094E-6295.09
GTAGCCT400.0295.01
ACCTAGG108.4534695E-4295.04
TACGCCT550.0295.01
GAGCCTA650.0295.02
GCCTAGG108.4534695E-4295.04
GGGCCCT400.0295.01
GACGCCT206.156915E-8295.01
GGCGCCT255.275069E-10295.01
CGCACCT600.0295.01
Produced by FastQC (version 0.11.5)