FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66150
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA26173.9561602418745276No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA23323.5253212396069538No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA19462.941798941798942No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17892.7044595616024187No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17462.639455782312925No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15552.3507180650037793No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14202.146636432350718No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13682.068027210884354No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13362.019652305366591No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13021.9682539682539684No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11791.7823129251700682No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11491.7369614512471654No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10851.64021164021164No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10221.5449735449735449No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9201.3907785336356764No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9081.3726379440665155No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7771.1746031746031746No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7721.167044595616024No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7391.1171579743008313No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7301.1035525321239608No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6751.0204081632653061No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5870.8873771730914588No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5640.8526077097505669No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5430.8208616780045351No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4880.7377173091458805No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4630.6999244142101285No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4440.6712018140589568No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4350.6575963718820862No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3700.5593348450491308No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3500.5291005291005291No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3490.527588813303099No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3360.5079365079365079No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3190.48223733938019653No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2800.4232804232804233No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2710.40967498110355255No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2570.3885109599395314No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2290.34618291761148906No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2280.34467120181405897No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2150.3250188964474679No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2120.3204837490551776No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2100.31746031746031744No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2060.31141345427059713No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2040.30839002267573695No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2040.30839002267573695No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1960.2962962962962963No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1940.2932728647014361No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.26152683295540435No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1690.25547996976568405No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1690.25547996976568405No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1660.2509448223733938No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1630.24640967498110355No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1560.235827664399093No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1540.2328042328042328No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1540.2328042328042328No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1490.2252456538170824No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1450.21919879062736206No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1430.2161753590325019No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1420.2146636432350718No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1400.21164021164021166No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1390.21012849584278157No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1370.2071050642479214No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1370.2071050642479214No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1370.2071050642479214No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1360.2055933484504913No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1350.20408163265306123No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1350.20408163265306123No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1330.20105820105820105No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1320.19954648526077098No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1300.19652305366591077No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1280.19349962207105062No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1240.1874527588813303No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1230.18594104308390022No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1230.18594104308390022No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1220.18442932728647013No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1200.18140589569160998No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.17989417989417988No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1170.17687074829931973No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1160.17535903250188967No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1150.17384731670445955No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1130.1708238851095994No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1100.16628873771730915No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1080.163265306122449No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.15873015873015872No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1020.15419501133786848No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1010.15268329554043839No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.15117157974300832No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA990.14965986394557823No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.14663643235071805No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA960.14512471655328799No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG950.1436130007558579No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT940.1421012849584278No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.1421012849584278No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA920.13907785336356765No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT920.13907785336356765No Hit
GGACTACTGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA890.1345427059712774No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA880.1330309901738473No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT880.1330309901738473No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA850.12849584278155707No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.1254724111866969No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT830.1254724111866969No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG820.12396069538926682No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA820.12396069538926682No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG790.11942554799697658No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA780.1179138321995465No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA780.1179138321995465No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA770.1164021164021164No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA770.1164021164021164No Hit
GGACTACCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA760.11489040060468632No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA730.11035525321239606No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA730.11035525321239606No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA720.10884353741496598No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT720.10884353741496598No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA700.10582010582010583No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA700.10582010582010583No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT700.10582010582010583No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.10430839002267572No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT690.10430839002267572No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG680.10279667422524565No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG670.10128495842781557No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGG108.4470346E-4295.0295
ACGGGTA1900.0295.08
AGTTAGG206.141272E-8295.0295
CTGGGTA108.4470346E-4295.07
GGACTAC62050.0294.286871
TACTCGG11250.0293.68895
CTACCCG7400.0293.006744
ACTCGGG11150.0292.354256
TACTGGG8150.0291.380375
TACACGG6100.0290.163945
ACACGGG6050.0290.123966
GACTACA16750.0289.716432
CTACCGG5000.0289.14
GACTACC17400.0289.06612
ACCCGGG7400.0289.020266
TACTAGG9500.0288.789465
TCGGGTT6850.0288.540168
ACTACAA5850.0287.435883
CTACAAG5850.0287.435884
CAAGGGT5800.0287.37077
Produced by FastQC (version 0.11.5)