FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134018
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC2020.1507260218776582No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC2010.1499798534525213No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1940.14475667447656285No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1890.14102583235087823No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1850.13804115865033056No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1840.13729499022519365No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1810.13505648494978287No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1800.13431031652464595No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1780.1328179796743721No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1760.13132564282409825No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1760.13132564282409825No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1720.12834096912355056No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1710.12759480069841364No Hit
CGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1700.12684863227327672No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1680.12535629542300286No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1680.12535629542300286No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1670.12461012699786596No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1630.12162545329731826No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1630.12162545329731826No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1620.12087928487218136No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1620.12087928487218136No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1600.1193869480219075No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1600.1193869480219075No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1590.11864077959677058No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1590.11864077959677058No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1590.11864077959677058No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1590.11864077959677058No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1590.11864077959677058No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1580.11789461117163366No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1570.11714844274649674No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1560.11640227432135981No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1560.11640227432135981No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1550.1156561058962229No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1550.1156561058962229No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1550.1156561058962229No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1550.1156561058962229No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1550.1156561058962229No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1550.1156561058962229No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1550.1156561058962229No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1540.11490993747108598No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1540.11490993747108598No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1530.11416376904594906No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1530.11416376904594906No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1520.11341760062081212No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1520.11341760062081212No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1520.11341760062081212No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1510.11267143219567521No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1510.11267143219567521No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1510.11267143219567521No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1510.11267143219567521No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1500.1119252637705383No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1500.1119252637705383No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1490.11117909534540138No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1480.11043292692026443No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1480.11043292692026443No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1480.11043292692026443No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1480.11043292692026443No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1470.10968675849512752No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1460.1089405900699906No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1460.1089405900699906No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1460.1089405900699906No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1450.10819442164485367No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1450.10819442164485367No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1450.10819442164485367No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1440.10744825321971675No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1440.10744825321971675No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1430.10670208479457984No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1430.10670208479457984No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1430.10670208479457984No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1430.10670208479457984No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1420.10595591636944292No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1420.10595591636944292No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1420.10595591636944292No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1410.10520974794430597No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1410.10520974794430597No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1400.10446357951916907No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1400.10446357951916907No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1400.10446357951916907No Hit
TAGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1390.10371741109403215No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1390.10371741109403215No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1390.10371741109403215No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1390.10371741109403215No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1380.10297124266889524No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1380.10297124266889524No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1370.10222507424375829No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1370.10222507424375829No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1370.10222507424375829No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1370.10222507424375829No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1370.10222507424375829No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1370.10222507424375829No Hit
GGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1370.10222507424375829No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1360.10147890581862137No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1360.10147890581862137No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1350.10073273739348446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCT700.0295.000031
GATCCCT350.0295.000031
CCTACCT350.0295.000031
GCACCCT700.0295.000031
TTGACCT350.0295.000031
GTTCCCT350.0295.000031
TCTCCCT350.0295.000031
CACCCCT700.0295.000031
TGGTCCT700.0295.000031
ACGACCT350.0295.000031
CGGACCT700.0295.000031
TGATCCT450.0295.01
TCGCCTA1850.0295.02
GCCCCTA1650.0295.02
AATCCTA2550.0295.02
GTCGCCT500.0295.01
CAAACCT550.0295.01
AGATCCT108.4566994E-4295.01
CGAACCT1000.0295.01
CTTCCTA2050.0295.02
Produced by FastQC (version 0.11.5)