FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34113
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA26957.900213994664791No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA15314.488025093073022No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA14644.291619030867998No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA10463.066279717409785No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA10032.940228065546859No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8982.632427520300179No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8932.6177703514789084No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8922.6148389177146543No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8752.5650045437223348No Hit
GGACTACAAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA7732.2659982997684165No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5141.5067569548266058No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4711.3807053029636795No Hit
GGACTACACGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4681.3719110016709173No Hit
GGACTACTAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4641.3601852666139007No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4121.2077507108726877No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA3561.0435904200744583No Hit
GGACTACTCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3451.0113446486676634No Hit
GGACTACCCGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3050.8940872980974994No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2930.8589100929264503No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2890.8471843578694339No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2870.8413214903409257No Hit
GGACTACCGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2860.8383900565766716No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2830.8295957552839094No Hit
GGACTACAGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2780.8149385864626388No Hit
GGACTACTGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2610.7651042124703192No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2590.7592413449418111No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2590.7592413449418111No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2510.7357898748277782No Hit
GGACTACCAGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2500.7328584410635242No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2440.7152698384779996No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2410.7064755371852374No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2020.5921496203793276No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1860.545246680151262No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1790.5247266438014833No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1790.5247266438014833No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1740.5100694749802128No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1710.5012751736874506No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1710.5012751736874506No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1690.49541230615894233No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1690.49541230615894233No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1620.47489226980916366No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1610.4719608360449096No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1600.4690294022806555No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1580.46316653475214725No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1570.4602351009878932No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1560.4573036672236391No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1560.4573036672236391No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1540.45144079969513085No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1510.4426464984023686No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1500.43971506463811444No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1480.4338521971096064No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1460.42798932958109814No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1460.42798932958109814No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1400.41040072699557356No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1360.39867499193855716No Hit
GGACTACAAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1360.39867499193855716No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1350.395743558174303No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1340.392812124410049No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1330.38988069064579484No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1230.3605663530032539No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1210.3547034854747457No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1130.3312520153607129No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1100.32245771406795065No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1100.32245771406795065No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1070.31366341277518833No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1050.30780054524668016No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1030.3019376777181719No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.2960748101896638No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.2755547738398851No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA880.2579661712543605No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA870.2550347374901064No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA860.2521033037258523No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.24624043619734412No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA840.24624043619734412No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA820.24037756866883592No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA820.24037756866883592No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA820.24037756866883592No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA800.23451470114032774No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA790.23158326737607363No Hit
GGACTACACGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA780.22865183361181954No Hit
GGACTACTAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA780.22865183361181954No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.2169260985548032No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA720.21106323102629498No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA710.20813179726204087No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA650.1905431946765163No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA630.1846803271480081No Hit
GGACTACTCGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA610.1788174596194999No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA610.1788174596194999No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA600.1758860258552458No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA590.1729545920909917No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA530.15536598950546712No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA530.15536598950546712No Hit
GGACTACCGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA500.14657168821270483No Hit
GGACTACCAGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA460.13484595315568845No Hit
GGACTACTGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA450.13191451939143434No Hit
GGACTACCCGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA440.12898308562718025No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.12312021809867206No Hit
GGACTACAGGGGTTTCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCA410.12018878433441796No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA390.11432591680590977No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA350.10260018174889339No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA350.10260018174889339No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAGG2350.0295.000035
CTACCAG2350.0295.000034
CCAGGGT2350.0295.000037
CGGGGTA2100.0295.08
GGACTCA108.429109E-4295.01
GTACTAC108.429109E-4295.01
AGGGTTT2500.0295.09
AATTAGC206.097798E-8295.0295
AAGGGTT1450.0295.08
TACACGG3700.0295.05
CTAGGGT4000.0295.07
CGGGTTT1200.0295.09
TGGGGTT400.0295.08
GGTTAGC157.17904E-6295.0295
TACCGGG2400.0295.05
ACGGGTT600.0295.08
GTTAGCC650.0295.0295
ACTGGGG2100.0295.06
TACTCGG2500.0295.05
AGTTCGC350.0295.0295
Produced by FastQC (version 0.11.5)