FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70033
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11137606556908886No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.11137606556908886No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10566447246298173No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10423657418645496No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.1013807776334014No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1013807776334014No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.1013807776334014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA1250.0295.000032
CTACCTA1250.0295.000032
ACCCCTA1250.0295.000032
CGCCCTA1250.0295.000032
GTCGCCT350.0295.01
AGATCCT450.0295.01
GCGACCT400.0295.01
TCACCCT450.0295.01
ATCTACG108.4480847E-4295.03
CACGGGA450.0295.06
CAGCCTA900.0295.02
CTATCCT500.0295.01
ATTACCT350.0295.01
CTCCCTA900.0295.02
GGTCCTA1000.0295.02
GGTCCCT206.1438186E-8295.01
CACGCCT500.0295.01
AGGACCT255.2568794E-10295.01
GGATCCT255.2568794E-10295.01
GCGCCTA900.0295.02
Produced by FastQC (version 0.11.5)