FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86901
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA1300.0295.02
CGAACCT550.0295.01
CGCGCCT350.0295.01
GCGCCTA900.0295.02
GCGCCCT206.152186E-8295.01
ACGGCGG157.2174007E-6295.08
CTACGGA2900.0295.06
CTCGCCT303.6379788E-12295.01
CCGACCT255.275069E-10295.01
GGACCTA950.0295.02
GGCGCCT255.275069E-10295.01
CGATCCT255.275069E-10295.01
CGGACCT157.2174007E-6295.01
TCGCCTA1050.0294.999972
GGGCCTA1050.0294.999972
TCCTACG23300.0290.568664
CCTACGG82550.0290.175665
GCCTACG16650.0289.684664
CTACGGG79900.0288.723426
CGCCTAC4700.0288.72343
Produced by FastQC (version 0.11.5)