FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108174
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1100.1016880211511084No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1100.1016880211511084No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCC1090.10076358459518923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT450.0295.01
GCCCCTA1100.0295.02
TCGCCCT305.456968E-12295.01
GCCCCCT305.456968E-12295.01
CTGTCCT650.0295.01
GTCGCCT400.0295.01
TGCCCCT450.0295.01
AGATCCT450.0295.01
CGAACCT255.275069E-10295.01
CACGGGA255.275069E-10295.06
TTGTCCT600.0295.01
GGTCCCT400.0295.01
ATAGCCT350.0295.01
CGCGCCT305.456968E-12295.01
GGATCCT700.0295.01
GCGCCTA1250.0295.02
CCTACGA255.275069E-10295.05
GCGCCCT550.0295.01
GTCTACG108.454447E-4295.02
TAGCCTA1400.0295.02
Produced by FastQC (version 0.11.5)