FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119214
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT400.0295.01
GTCTACG157.2241255E-6295.03
TATTCCT400.0295.01
CTACCGG108.4555295E-4295.06
CGGTCCT206.1618266E-8295.01
GGCGCCT157.2241255E-6295.01
GCTACGG108.4555295E-4295.04
GTGCCTA1100.0295.02
TACGGGA109900.0287.886727
CGACCTA1750.0286.57142
GTTCCTA1700.0286.323552
ACGGGAG110350.0286.311748
CGGGAGG107800.0284.190649
CTACGGG112850.0283.89016
GCGCCTA1250.0283.199982
CGCCTAC5750.0282.173923
CCTACGG116500.0281.57945
TCCCTAC7300.0280.856143
GGCCTAC6150.0280.609743
CCCCTAC7150.0280.559453
Produced by FastQC (version 0.11.5)