FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60311
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCT206.1367246E-8295.01
ATAGCCT255.2568794E-10295.01
CGCGCCT157.2064577E-6295.01
GCGCCTA500.0295.02
CCTACCG108.445184E-4295.05
TAGCCTA900.0295.02
CCGTCCT350.0295.01
GCACCCT108.445184E-4295.01
TTCCCTA850.0295.02
TACGCCT157.2064577E-6295.01
TGCGCCT206.1367246E-8295.01
CTACGAG350.0295.06
TCGCTTT350.0295.0295
CCCCCTA500.0295.02
CTACCGG108.445184E-4295.06
CCGACCT303.6379788E-12295.01
GACGCCT303.6379788E-12295.01
ACGAGGC206.1367246E-8295.08
CCACGGA108.445184E-4295.05
CGATCCT206.1367246E-8295.01
Produced by FastQC (version 0.11.5)