FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68594
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA23253.389509286526518No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA18842.7465959121789076No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA14692.141586727702131No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA14422.1022246843747268No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13772.007464209697641No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13161.918535148846838No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA12371.803364725777765No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12351.8004490188646238No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12121.7669183893635012No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA11461.670700061229845No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9921.4461906289179811No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA9491.3835029302854476No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9311.357261568067178No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA9171.33685161967519No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA9061.3208152316529143No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9041.3178995247397731No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA8761.2770796279557979No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8191.1939819809312768No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7991.164824911799866No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7901.151704230690731No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7851.1444149634078784No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7641.1138000408198967No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA7441.0846429716884858No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7301.0642330232964983No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6901.0059188850336764No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6580.959267574423419No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA6420.9359419191182902No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6000.8747120739423273No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5930.8645070997463336No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA5810.8470128582674868No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5760.8397235909846342No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA5550.8091086683966529No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA5290.7712044785258186No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5160.7522523835904015No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5120.7464209697641193No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5070.7391317024812666No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA4950.7216374610024201No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA4870.7099746333498557No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4730.689564684957868No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4670.6808175642184447No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA4630.6749861503921626No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4380.6385398139778989No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA4080.5948042102807826No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3950.5758521153453655No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3660.5335743651048197No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3560.5189958305391141No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA3530.5146222701694025No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA3400.49567017523398543No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA3370.49129661486427384No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3230.47088666647228616No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA3110.4533924249934397No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3040.4431874507974458No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2980.4344403300580226No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2930.4271510627751699No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA2870.41840394203574655No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2770.40382540747004114No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA2560.37321048488205966No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA2400.34988482957693096No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2310.336764148467796No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA2250.32801702772837277No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA2080.3032335189666735No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA2030.2959442516838207No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2020.2944863982272502No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1960.2857392774878269No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1950.28428142403125634No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1940.2828235705746858No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1900.27699215674840366No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1870.272618596378692No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1810.26387147563926877No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1770.25804006181298655No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1750.2551243548998455No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1700.24783508761699272No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1660.24200367379071056No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1620.23617225996442837No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1570.22888299268157566No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1550.22596728576843458No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCA1540.224509432311864No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1530.22305157885529345No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1520.2215937253987229No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1500.21867801848558183No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1490.2172201650290113No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.21576231157244075No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1410.20555733737644694No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1380.20118377700673531No Hit
GGACTACAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1360.19826807009359418No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.1953523631804531No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1300.1895209493541709No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1290.18806309589760037No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.18660524244102983No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1270.18514738898445926No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1260.18368953552788872No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1210.17640026824503602No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1210.17640026824503602No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCA1210.17640026824503602No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1200.17494241478846548No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1170.17056885441875383No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1160.1691110009621833No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1150.16765314750561275No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1130.16473744059247164No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1130.16473744059247164No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1120.1632795871359011No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1110.16182173367933056No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1110.16182173367933056No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.16036388022276No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCA1070.15599031985304837No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1020.14870105257019564No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA1010.1472431991136251No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1010.1472431991136251No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA1000.14578534565705456No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA980.14286963874391345No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.1370382249176313No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCA930.13558037146106072No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCA920.13412251800449018No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA920.13412251800449018No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA920.13412251800449018No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA900.1312068110913491No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA870.12683325072163745No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCA870.12683325072163745No Hit
GGACTACTCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA850.12391754380849636No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA850.12391754380849636No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.12245969035192582No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA840.12245969035192582No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA830.12100183689535528No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA790.1151704230690731No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCCTCA790.1151704230690731No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.1151704230690731No Hit
GGACTACCCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA790.1151704230690731No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.11371256961250256No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.11371256961250256No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA770.112254716155932No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA760.11079686269936145No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA750.10933900924279091No Hit
GGACTACCGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA720.10496544887307928No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA710.10350759541650874No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCATGA710.10350759541650874No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA690.10059188850336766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTA5350.0295.08
ACTCGGG5950.0295.06
ACTTGGG108.4477034E-4295.06
GGACTCC108.4477034E-4295.01
GGACTAC65550.0295.01
AATTATC400.0295.0295
AATTAGT157.2107596E-6295.0295
AATTAGG108.4477034E-4295.0295
AATTAAC303.6379788E-12295.0295
ACTTCGC108.4477034E-4295.0295
CTTGGGT108.4477034E-4295.07
GGTAGCC108.4477034E-4295.0295
ATTTGCC157.2107596E-6295.0295
CTCGGGT5950.0295.07
TATTAGC108.4477034E-4295.0295
TAGGGTT2650.0295.08
TACTCGG5950.0295.05
CTACCGG5400.0295.04
CTACTAG6400.0295.04
AATTGGC157.2107596E-6295.0295
Produced by FastQC (version 0.11.5)