FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63927
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.14704272060318802No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.14547843634145197No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.12827130946235552No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.12670702520061944No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.12514274093888342No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.12357845667714737No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.12201417241541133No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.12044988815367529No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.12044988815367529No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.12044988815367529No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.11888560389193925No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.11575703536846715No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.11419275110673112No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11262846684499507No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11262846684499507No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11106418258325904No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11106418258325904No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11106418258325904No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11106418258325904No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.109499898321523No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.109499898321523No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.109499898321523No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.109499898321523No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.109499898321523No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10793561405978694No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10324276127457882No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10324276127457882No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10324276127457882No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10324276127457882No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10167847701284277No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10167847701284277No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10167847701284277No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10011419275110674No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10011419275110674No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10011419275110674No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10011419275110674No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10011419275110674No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAA108.4463623E-4295.0295
TGATCCT206.139635E-8295.01
TCGCCTA700.0295.02
AGTCCTA1000.0295.02
GTCGCCT206.139635E-8295.01
TGCCCCT157.2084713E-6295.01
ACGCCCT108.4463623E-4295.01
GCGACCT500.0295.01
TCCGCCT500.0295.01
CACGGGA157.2084713E-6295.06
CCCTAGG108.4463623E-4295.04
ACACCTA1800.0295.02
ACCGCCT206.139635E-8295.01
GATCCCT108.4463623E-4295.01
GCGCCTA450.0295.02
CCTACGA108.4463623E-4295.05
ACTTCCT350.0295.01
GCGCCCT206.139635E-8295.01
CGCGAAA108.4463623E-4295.0295
TAGCCTA900.0295.02
Produced by FastQC (version 0.11.5)