FastQCFastQC Report
Wed 8 May 2024
EGAF00005491797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76646
Total Bases23 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.13568875088067217No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.12655585418678078No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.12655585418678078No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.12525115465908201No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.12525115465908201No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.12394645513138325No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.12394645513138325No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.1213370560759857No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11611825796519061No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11611825796519061No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.11350885890979308No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10959476032669675No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10829006079899799No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10829006079899799No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10829006079899799No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10698536127129923No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10698536127129923No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10698536127129923No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10698536127129923No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10698536127129923No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10698536127129923No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.10568066174360044No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10568066174360044No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10437596221590167No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10437596221590167No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10437596221590167No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10307126268820292No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.10307126268820292No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.10307126268820292No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.10307126268820292No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10307126268820292No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.10176656316050413No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.10046186363280538No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.10046186363280538No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.10046186363280538No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)