FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41952
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC710.16924103737604881No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC710.16924103737604881No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC710.16924103737604881No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC710.16924103737604881No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC680.16209000762776507No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC660.15732265446224256No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC650.1549389778794813No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC650.1549389778794813No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC640.15255530129672007No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC640.15255530129672007No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC630.1501716247139588No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC630.1501716247139588No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC620.14778794813119756No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC620.14778794813119756No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC610.14540427154843633No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC610.14540427154843633No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC600.14302059496567504No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC600.14302059496567504No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC600.14302059496567504No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC600.14302059496567504No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC590.1406369183829138No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC590.1406369183829138No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC590.1406369183829138No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC590.1406369183829138No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC590.1406369183829138No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC590.1406369183829138No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC580.13825324180015255No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC580.13825324180015255No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC560.13348588863463007No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC560.13348588863463007No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC560.13348588863463007No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC550.13110221205186878No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC550.13110221205186878No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC550.13110221205186878No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC550.13110221205186878No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC540.12871853546910755No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC540.12871853546910755No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC540.12871853546910755No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC540.12871853546910755No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC540.12871853546910755No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC540.12871853546910755No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC540.12871853546910755No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC540.12871853546910755No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC530.1263348588863463No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC530.1263348588863463No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC530.1263348588863463No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC530.1263348588863463No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC530.1263348588863463No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC530.1263348588863463No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC520.12395118230358504No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC520.12395118230358504No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC520.12395118230358504No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC520.12395118230358504No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC520.12395118230358504No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC520.12395118230358504No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC520.12395118230358504No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC510.1215675057208238No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC510.1215675057208238No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC510.1215675057208238No Hit
CTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC510.1215675057208238No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC510.1215675057208238No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC510.1215675057208238No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC510.1215675057208238No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC510.1215675057208238No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC510.1215675057208238No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC500.11918382913806255No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC500.11918382913806255No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC500.11918382913806255No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC500.11918382913806255No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC500.11918382913806255No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC500.11918382913806255No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC500.11918382913806255No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC500.11918382913806255No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC490.1168001525553013No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC490.1168001525553013No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC490.1168001525553013No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC490.1168001525553013No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC490.1168001525553013No Hit
GACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC480.11441647597254005No Hit
TGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC480.11441647597254005No Hit
ACAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC480.11441647597254005No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC480.11441647597254005No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC480.11441647597254005No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC470.11203279938977881No Hit
GTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC470.11203279938977881No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC470.11203279938977881No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC470.11203279938977881No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC460.10964912280701754No Hit
CTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC460.10964912280701754No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC460.10964912280701754No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC460.10964912280701754No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC460.10964912280701754No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC460.10964912280701754No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC460.10964912280701754No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
TGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
CGCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC450.1072654462242563No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC440.10488176964149505No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC440.10488176964149505No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC440.10488176964149505No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC440.10488176964149505No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC440.10488176964149505No Hit
CCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC440.10488176964149505No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC440.10488176964149505No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
GTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC430.10249809305873378No Hit
AAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC420.10011441647597254No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC420.10011441647597254No Hit
GCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC420.10011441647597254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA350.0295.02
GCCCCTA650.0295.02
ACAACCT157.19084E-6295.01
AGTCCTA450.0295.02
GTCGCCT157.19084E-6295.01
CGAACCT157.19084E-6295.01
GCGACCT108.436031E-4295.01
TTAGCCT206.114533E-8295.01
AGCCCTA255.2204996E-10295.02
TAGACCT108.436031E-4295.01
CCCTACG9950.0295.04
CGGCCCT206.114533E-8295.01
GGCACCT303.6379788E-12295.01
ACACCCT157.19084E-6295.01
GGTCCTA450.0295.02
CAACCTA850.0295.02
ATAGCCT206.114533E-8295.01
CACGCCT157.19084E-6295.01
TGAACCT255.2204996E-10295.01
GCGCCCT206.114533E-8295.01
Produced by FastQC (version 0.11.5)